FIG. 10.

Dominant negative TCF4 inhibits leukemic cell growth and plakoglobin affects hematopoietic progenitors. (a) AML1-ETO-positive Kasumi-1 cells were transfected with a dominant negative TCF4 expression vector. In subsequent triplicate colony assays with neomycin selection, dnTCF4-containing Kasumi-1 cells showed reduced clonal growth. The mean and standard error of two independent experiments are shown. (b) ScaI⁺ lin⁻ mouse bone marrow cells were transduced with the indicated retroviral vectors; 15 days after seeding in methylcellulose, the expression of the stem cell antigen ScaI or the differentiation marker Mac1 of the green fluorescent protein-positive cells was determined by flow cytometry. The results of one representative experiment out of three are shown.