FIG. 2.
Regulation of the shared target genes by vitamin D3, TSA, and expression in Kasumi-1 cells. (a) This scatter plot depicts the association between regulation by the fusion proteins (mean expression levels in fusion protein samples/mean expression levels in control) and gene regulation by vitamin D3 exposure (mean expression fusion proteins + vitamin D3/mean expression fusion proteins). Values above 1 indicate induction, and values lower than 1 indicate repression. Dotted lines indicate a twofold induction or repression level. Genesfound to be repressed by the fusion proteins are shown in green, and induced genes are indicated as red symbols. (b) Similar analyses were performed for the effect of the histone deacetylase-inhibitor TSA on gene expression in the fusion protein-expressing samples. TSA-induced genes show levels higher than 1 on the y axis. (c) Expression levels of the fusion protein-regulated genes in Kasumi-1 cells with the average intensity units as calculated by the Affymetrix software are shown on the x axis. Gene regulation by TSA in Kasumi-1 cells is indicated on the y axis. Values on the y axis higher than 1 indicate induction of the gene by TSA in Kasumi-1 cells. More than 33% of the repressed genes were induced more than twofold by TSA in Kasumi-1 cells. Genes found to be repressed by the fusion proteins are shown in green, and induced genes are indicated as red symbols.