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. 2013 May 23;2(7):667–674. doi: 10.1242/bio.20134531

Fig. 5. LIM domain requirement for transient paxillin accumulation at damaged SFs.

Fig. 5.

(A) Domain structure of paxillin. Paxillin is a 557-amino acid (human), 68-kDa protein comprising multiple structural domains including 5 leucine-rich LD motifs and 4 double zinc finger LIM domains. The deletion mutants lack either the 5 LD motifs (Pxn-C) or the 4 LIM domains (Pxn-N). (B) The micrographs and kymographs are of AcGFP1-actin and TagRFP-Pxn-N or TagRFP-Pxn-C in rat fibroblasts. Scale bars: 20 µm. (C) Distributions of the kinetic parameters for accumulation and dissociation of full length paxillin and Pxn-C during repair of damaged stress fibres as box plots created as described in Fig. 2. All parameters were estimated from the kymographs (full length paxillin: 16 cells, 33 SFs; Pxn-C: 13 cells, 35 SFs).