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. 2013 May 23;2(7):686–694. doi: 10.1242/bio.20134747

Fig. 4. The C-terminus is sufficient and necessary for VPAC2 ciliary targeting.

Fig. 4.

(A) VPAC2-GFP (but not VPAC1-GFP and PAC1-GFP) targets to primary cilia in IMCD3 cells. IMCD3 cells were transduced with lentivirus vectors expressing VPAC2-GFP, VPAC1-GFP or PAC1-GFP and were serum starved to induce ciliogenesis. Cells were immunostained for acetylated α-tubulin (AcTubulin, red). (B) Schematic of CD8α chimeras. (C–G) The last 44 amino acids of VPAC2's C-terminus are sufficient to target the non-ciliary membrane protein CD8α to primary cilia. IMCD3 cells expressing GFP-tagged CD8α, CD8α-VPAC2-Cterm, CD8α-VPAC2-Cterm44, CD8α-PAC1-Cterm and CD8α-VPAC1-Cterm were serum starved and immunostained for AcTubulin (red). DNA was labeled with DAPI (C–G). Insets show an unmerged image of the region around the cilium. Scale bars: 5 µm.