Fig. 4. transparent encodes Mpv17.

(A) tra maps to chromosome 20. The genomic interval was mapped by the indicated microsatellites (z-marker) and further refined by SNPs located on contigs BX596783 and BX511178. Brackets give the number of recombinants in relation to the total number of mutant individuals. In this closest interval, 7 genes are annoted. (B) Sequencing revealed a deletion in the mpv17 coding sequence in tra mutants, resulting in a frameshift (D, amino acids in pink) and an early stop codon. (C) Comparison of zebrafish (Dr) Mpv17 protein sequence and its human homolog (Hs). The proteins show 69% amino acid identity. Amino acids forming the transmembrane domains are highlighted in red. The mitochondrial target signal in the human protein sequence is underlined. (D) Injection of mpv17 or mpv17:egfp encoding mRNA into tra mutants rescue larval iridophore pigmentation (n(uninjected) = 167, n(mpv17) = 299, n(mpv17:egfp) = 300).