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. Author manuscript; available in PMC: 2014 Apr 18.
Published in final edited form as: Chem Biol. 2013 Apr 18;20(4):604–613. doi: 10.1016/j.chembiol.2013.04.001

Figure 1.

Figure 1

Development of an Electrophoretic Mobility Shift Assay for Protein Sumoylation. (A) Sequence and reactivity of a fluorescent polypeptide substrate for the sumoylation assay. (B) In-gel fluorescence and (C) Western blot (with anti-SUMO-1 antibody) experiments showing the sumoylation of the fluorescent peptide. (D) Separation of the substrate peptide and sumoylated product using the LabChip EZ Reader II system. (E) Kinetic measurement of fluorescent peptide sumoylation. A sample from one 30 µL reaction mixture treated with 0.1% DMSO (either with or without UBC9) was analyzed using the LabChip EZ Reader II system every 4.88 minutes for 5 hours and percent conversion was monitored at each time point.