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. Author manuscript; available in PMC: 2014 Apr 18.
Published in final edited form as: Chem Biol. 2013 Apr 18;20(4):604–613. doi: 10.1016/j.chembiol.2013.04.001

Figure 2.

Figure 2

Ten Most Active Compounds from the Kinetic Screen. GA30 = Ginkgolic acid C15, 30 µM. All other compounds are assayed at 5 µM. The sumoylation reaction was performed for 90 minutes at room temperature, and the reaction was quenched by the addition of EDTA. Percent conversion was measured using the LabChip EZ Reader II system as described in the Experimental Procedures. Percent conversion is normalized to a DMSO-treated control sample in which percent conversion is 100. Values represent the mean of three replicates.