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Structure-Activity Relationship of 2-D08. Sumoylation reactions were performed for 2 hours at room temperature, and then were quenched by the addition of EDTA. Percent conversion was measured using the LabChip EZ Reader II system as described in Experimental Procedures. Percent conversion is normalized relative to a DMSO-treated control sample in which percent conversion is 100. Values represent the mean of three replicates.
