Table 2.

Number of Different Nuclear Classes 28 Days After Light-Induced Damage

	Average Number of Nuclei Per Defined Linear Distance
	GCL	p-Value	INL	p-Value	Müller Glia	p-Value	ONL	p-Value
Uninjected control	48 (5)	0.82	319 (15)	0.073	34 (1)	0.0001	161 (9)	0.0002
PCNA MO	50 (8)		281 (6)		16 (1)		52 (11)

The number of nuclei in the ganglion cell layer (GCL), inner nuclear layer (INL), and outer nuclear layer (ONL) were quantified from frozen sections that were stained with TO-PRO-3, a nuclear stain. The number of Müller glial nuclei was quantified from glial fibrillary acidic protein (GFAP) immunolocalization on frozen sections. Both control and PCNA morphant retinas were treated in constant light for 4 days and returned to standard 14 h-light:10-h dark cycle for 28 days before analysis. Three sections (either containing the optic nerve or an adjacent section) from eight different eyes were quantified for each measurement. The number of nuclei was detemined per defined linear distance of 370 in the central dorsal retina. Numbers in parentheses represent the standard error of the mean. p values from statistical comparison across each group (GCL, INL, Muüller glia, and ONL) was determined by ANOVA.