FIG. 3.

S. cerevisiae L868F DNA pol α mutation spectrum in M13mp2 lacZα. Mutation spectra were determined by sequencing 135 (WT pol α) and 137 (L868F pol α) mutant plaques. The M13mp2 DNA sequence is shown. Base substitutions (A) and frameshifts (B) produced by S. cerevisiae L868F are indicated above the M13mp2 sequence, and those produced by WT pol α are indicated below the sequence. The +1 and −1 frameshift mutations are indicated by Δ (deletion) and + (insertion), respectively. For a frameshift in a run sequence, the symbol is centered under the run. Endpoints of large deletions are indicated by one arrow pointing down and one arrow pointing up. The nucleotide position at the corresponding other endpoint is indicated at the arrow.