TABLE 6.
Mutation rate of S. cerevisiae strains that carry either WT or mutant pol1a
| pol1 | MMR mutation rate forb:
|
|||||
|---|---|---|---|---|---|---|
| CAN1r WT (10−7) | his+ WT (10−8) |
trp+ (10−8)
|
||||
| WT | ΔMSH2 | ΔMSH3 | ΔMSH6 | |||
| WT | 4.8 (1)b | 2.1 (1) | 5.7 (1) | 27 (1) | 2.1 (1) | 36 (1) |
| L868F | 39 (8.1)d | 15 (7.1)d | 83 (15)d | -c | 24 (11) | -c |
| L868M | 14 (2.9)d | 2.6 (1.2) | 8.4 (1.5) | 1,400 (52)d | 4.4 (2.1) | 2,200 (61)d |
| L868W | 4.8 (1.0) | 3.2 (1.5) | 5.0 (0.9) | 400 (15) | 2.0 (1.0) | 170 (4.7)d |
a
Experiments were carried out (using independent clones) more than five times.
b
The numbers in parentheses represent severalfold induction values relative to the WT value.
c
-, double mutant was not successfully created.
d
Statistically relevant (Student t test; P < 0.05).