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. Author manuscript; available in PMC: 2013 Jul 15.
Published in final edited form as: J Cell Sci. 2008 Apr 22;121(0 10):1641–1648. doi: 10.1242/jcs.014076

Fig. 1.

Fig. 1

Co-immunoprecipitation of αIIbβ3 with SFKs from platelets. (A) Equal amounts of total protein in the Triton-X-100-soluble extract of resting platelets or thrombin-treated (aggregated) platelets were subjected to immunoprecipitation using indicated anti-SFK antibodies. The immunoprecipitates were subjected to immunoblot analysis using anti-β3 antibodies. AP3 antibody (lane 2; anti-β3 antibody; positive control) but not control Ig (lane 1; normal mouse serum) immunoprecipitated β3 integrin (bottom panel). (B) Densitometric analysis (n=3) of blots in panel A. (C) Anti-SFK antibodies immunoprecipitated equal amounts of Src and Fyn from resting and thrombin-treated platelets.