Fig. 3.

Fyn associates with both the full-length and the deletion mutant of αIIbβ3. (A,B) CHO cells stably expressing either full-length or truncated β3-subunits, together with the full-length αIIb subunit, were transiently transfected with the cDNAs encoding (A) GFP-Src or (B) GFP-Fyn. 48 hours after transfection, cells were re-plated on fibrinogen-coated plates for 1 hour. Cell extracts were subjected to immunoprecipitation using antibodies against the β3 subunit. The immunoprecipitates were then analyzed for (A) Src or (B) Fyn using anti-GFP antibodies. Expression levels of transfected integrins and GFP fusions are also shown.