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Thermal unfolding of αTm homo- and heterodimers carrying HCM mutations compared to WT-Tm. Normalized unfolding profiles of 7 μM homodimers (solid lines) and heterodimers (dashed lines) of D175N (A and B) and E180G (C and D) in the presence (B and D) or absence (A and C) of 1 mM DTT. Buffer conditions: 0.5 M KCl, 5 mM MgCl2, 20 mM potassium phosphate buffer (pH 7.0). The CD spectrum of 7 μM αD175N-Tm in the range of 195–250 nm is shown in the inset of panel A.
