Fig. 4. TCRs from colonic tTregs recognize microbial antigens.

a. The response (± s.e.m from three experiments) of cloned colonic Treg hybridomas, which responded to cecal lysate from untreated TCR^miniFoxp3^GFP mice (Fig. S6), to re-stimulation with cecal lysate from untreated or antibiotic-treated mice. b, The abundance of Treg TCRs from hybridomas that responded to cecal lysates from untreated mice (shown in a) on CD4⁺Foxp3⁺ thymocytes. Hybridomas marked by asterisk also responded to cecal lysate from antibiotic-treated mice. c, Phylogenetic distance of bacterial strains tested here. d. The response of hybridomas highlighted in panel 4b to indicated bacterial sonicates.