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(A) Proposed mechanism of reaction of HPr+ with O2•−. (B) Scheme showing the oxidation of HE, HPr+ and Mito-HE probes by O2•− to the hydroxylated fluorescent product and by one-electron oxidants with the formation of fluorescent, non-hydroxylated cation and non-fluorescent dimeric products. For the sake of clarity, the structures of other intermediate dimeric products are not shown.
