FIG. 5.

Oxidative stress-induced association between PKCδ and PKD. HeLa cells were transfected with the indicated mutant PKDs, and PKD was immunoprecipitated (IP; anti-HA antibody). The immunoprecipitates were resolved by SDS-PAGE and immunoblotted for coimmunoprecipitated endogenous PKCδ. Immunoblots were reprobed to evaluate equivalent PKD immunoprecipitation. Immunoblot assays of cell lysates were also used to monitor endogenous expression of PKCδ. All results are typical of three independent experiments.