FIG. 7.

Oxidative stress-mediated PKD phosphorylation is blocked by PKCδ RNAi. HeLa or HEK 293E cells were transfected with a pSUPER-PKCδ RNAi construct or a control vector. After 48 h, cells were stimulated with H₂O₂ (10 μM, 10 min). Endogenous PKD was immunoprecipitated (IP; anti-PKD antibody) and analyzed for activation loop phosphorylation (anti-pS738/742 antibody). The blot was reprobed against PKD. Silencing of endogenous PKCδ was monitored by immunoblot analysis. All results are typical of three independent experiments.