Table 3.
QRT-PCR validation of selected candidate genes from micropunched tissue and IL projection neurons cells
| Gene | Gene title | Microarray bulk |
qRT-PCR bulk |
qRT-PCR IL neurons |
|||
|---|---|---|---|---|---|---|---|
| p | FC | p | FC | p | FC | ||
| Egr1 | Early growth response 1 | 0.0231 | −0.2524 | 0.0137 | −0.5738 | 0.196 | −1.294 |
| Egr2 | Early growth response 2 | 0.0084 | −0.7276 | 0.0123 | −0.8669 | 0.006 | −9.844 |
| Egr4 | Early growth response 4 | 0.0006 | −0.5907 | 0.0077 | −0.7916 | 0.041 | −1.203 |
| Gria3 | Ionotrophic glutamate receptor 3 | 0.0356 | −0.3008 | 0.0368 | −0.3686 | 0.683 | −0.316 |
| Grm2 | Metabotropic glutamate receptor 2 | 0.0044 | −0.2747 | 0.0102 | −0.5101 | 0.004 | −2.402 |
| Crym | Mu-crystallin homolog | 0.0065 | −0.2962 | 0.0292 | −0.6153 | 0.161 | −0.897 |
| Nr4a1 | Nuclear receptor subfamily 4, group A, member 1 | 0.0074 | −0.2802 | 0.0063 | −0.7108 | 0.001 | −2.402 |
| Nr4a3 | Nuclear receptor subfamily 4, group A, member 3 | 0.0061 | −0.3224 | 0.0005 | −1.2155 | Nondetectable | |
| Slc1a3 | Glial high affinity glutamate transporter | 0.7954 | −0.0163 | Not assessed | Nondetectable | ||
Validation of selected genes determined to be differentially expressed in the mPFC exposed group (n = 9) versus control (n = 9) by microarray analysis or in the IL of the exposed group (n = 5) versus control (n = 5) is shown. Bold values are confirmed qRT-PCR data from microarray results. FC, fold change.