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. 2013 Apr 17;33(16):6742–6752. doi: 10.1523/JNEUROSCI.0528-13.2013

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Copyright © 2013 the authors 0270-6474/13/336742-11$15.00/0

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Figure 6. — arg^+/−α3^+/− mice have decreased synapse density, disrupted synaptic ultrastructure, and abnormal behavior. A, Representative electron micrographs of hippocampal SC–CA1 synapses from P42 WT and arg^+/−α3^+/− mice. Scale bar, 200 nm. B, Mean synapse density is reduced in arg^+/−α3^+/− mice compared with WT littermates at P42 but not P21. ANOVA between groups: F = 8.983, p < 0.0001. Post hoc Student's t test: P21, p = 0.6427; P42, p < 0.0001. n = 33–52 sections from 3–4 mice per group. C, Mean PSD length is increased in arg^+/−α3^+/− synapses compared with WT littermates at P42. At P21, arg^+/−α3^+/− synapses have an increased PSD length compared with WT, although it was not statistically significant. ANOVA between groups: F = 5.218, p = 0.0015. Post hoc Student's t test: P21, p = 0.0689; P42, p = 0.0015. n = 75–112 synapses from 3–4 mice per group. D, Mean dendritic spine cross-sectional head area fails to decrease in arg^+/−α3^+/− synapses between P21 and P42 compared with WT littermates. ANOVA between groups: F = 6.519, p = 0.0003. Post hoc Student's t test: P21, p = 0.6215; P42, p = 0.0008. Additionally, the variance in WT spine head areas decreases between P21 and P42, but there is no difference in arg^+/−α3^+/− spine head area variance between the two ages. Two-sampled F test: WT, p < 0.0001; arg^+/−α3^+/−, p = 0.7255. n = 75–112 synapses from 3–4 mice per group. E, Performance of WT, arg^+/−, α3^+/−, arg^+/−α3^+/−, α5^+/−, and arg^+/−α5^+/− mice in an object recognition task at P42. On day 1, exploration time of two identical objects is identical in all genotypes. Here, all genotypes show a preference for the novel object (white bar) on day 3 of testing, except arg^+/−α3^+/− mice who spend equal time with the novel object and familiar object from day 1 (black bar). Three-way ANOVA (object × arg × integrin): F = 3.868, p = 0.023; main effect of object, F = 160.091, p < 0.001. Post hoc Student's t test: WT, p < 0.0001, n = 22 mice; arg^+/−, p < 0.0001, n = 17 mice; α3^+/−, p < 0.0001, n = 11 mice; arg^+/−α3^+/−, p = 0.0724, n = 8 mice; α5^+/−, p < 0.0001, n = 9 mice; arg^+/−α5^+/−, p < 0.0001, n = 9 mice. Error bars indicate mean ± SEM. *p < 0.05.