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. Author manuscript; available in PMC: 2013 Jul 15.
Published in final edited form as: Nat Immunol. 2010 Oct 31;11(12):1085–1092. doi: 10.1038/ni.1955

Figure 4.

Figure 4

CD8 memory responses are dependent on ZAP-70 catalytic activity. (a,b) Flow cytometric analysis of LCMV (a) GP33–41-specific and (b) NP396–404-specific IFNγ and TNFα production by Zap70+/− and Zap70(AS) memory CD8+ cells. Contour plots are gated on CD8+ CD44high memory cells. The concentrations of 3-MB-PP1 present are indicated above the plots. (c, d) Graphs display the percentage of IFNγ+ TNFα+ cells at each concentration of 3-MB-PP1 tested. Percentages are of CD8+ CD44high memory Zap70+/− (black line) and Zap70(AS) (gray line) cells. Error bars indicate the standard deviation from the mean; n=3. Data are representative of 3 experiments.