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. Author manuscript; available in PMC: 2013 Jul 15.
Published in final edited form as: Nat Immunol. 2010 Oct 31;11(12):1085–1092. doi: 10.1038/ni.1955

Figure 5.

Figure 5

ZAP-70 catalytic activity is dispensable for TREG cell suppressive activity in vitro. (a) In vitro suppression assays used CD4+CD25− Tconv cells from both Zap70+/− and Zap70(AS) mice, together with irradiated antigen-presenting cells, anti-CD3ε mAbs, and titrated numbers of Zap70+/− or Zap70(AS) CD4+CD25+ TREG cells. Cultures were set up in the presence of vehicle alone, 5 µM, or 10 µM 3-MB-PP1. Data are presented as the average 3H thymidine uptake in triplicate cultures. Error bars indicate the standard deviation of the mean. (b) Bar graphs show the 3H thymidine uptake of Tconv or TREG cells alone in the presence of vehicle alone or 3-MB-PP1. (c) Immunoblots of whole cell lysates from purified Zap70+/− and Zap70(AS) Tconv and TREG cells were probed for expression of Syk, ZAP-70, and ERK1-ERK2. Data in all panels are representative of at least 3 experiments.