Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Jul 15;126(14):3095–3104. doi: 10.1242/jcs.122788

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013. Published by The Company of Biologists Ltd

PMC Copyright notice

Fig. 3. — DNA damage in B spermatogonia of Ku70-deficient testes revealed by 53BP1 (red) and γH2AX staining (green). (A) Testicular section of a wild-type mouse showing a few 53BP1 foci (red) in B spermatogonia (indicated by letter B). Wild-type Sertoli cells (S) show no 53BP1 foci. XY denotes the 53BP1- and γH2AX-positive sex body (yellow) of pachytene spermatocytes. (B) Numerous 53BP1 DNA damage foci in B spermatogonia of a Ku70^−/−Rap1^Δ/Δ testis section. These colocalize with γH2AX foci (green) as shown by the green channel image of one nucleus in the inset. The white arrow denotes a large 53BP1 DNA damage focus in the DAPI-faint chromatin of a double knockout Sertoli cell nucleus. (C) γH2AX and 53BP1 foci numbers are significantly increased (**P<0.001) in Ku70-deficient B spermatogonia of single and double knockout testes relative to the Rap1^Δ/Δ, heterozygous and wild-type testes. Error bars indicate s.d.