Fig. 2.

Mass spectrometry analysis and validation of high MW complexes isolated from fch/fch mice. (A) Nuclear extracts from wild-type (+/+) and fch/fch (−/−) liver were subjected to 8% SDS-PAGE followed by staining of the gel with Coomassie Blue. Extracts from the fch/fch mice showed the presence of high MW bands (shown in the two red boxes). The boxed bands were excised and analyzed by mass spectrometry. The proteins that were identified by mass spectrometry were categorized as structural proteins, signaling mediators and transcription factors, nuclear pore proteins and ribosomal proteins. (B) Nuclear extracts from livers of wild-type, wt/fch (+/−) and fch/fch mice were analyzed by immunoblotting using antibodies to Nup155 and β-catenin. Brackets highlight the high MW species. (C) Liver sections from wild-type and fch/fch mice were stained for Nup proteins (green) and nuclei (blue) using mAb414 antibody. Scale bar: 5 µm.