Skip to main content
. 2013 Jul 15;126(14):3204–3213. doi: 10.1242/jcs.128587

Fig. 7.

Fig. 7.

CETN1 expression during centriole rearrangement in spermatids in late spermiogenesis. (A,B) Centrin expression was detected by immunofluorescence. Double-labeling of CETN1 (red) and the centriole marker CETN3 (green) in acrosome phase spermatids (steps 8–12) of Cetn1+/+ and Cetn1−/− mice. (A) CETN1 and CETN3 were colocalized in the centrioles of spermatid centrosomes, the distal and the proximal centrioles (arrows) of the sperm flagellar basal apparatus as well as the centriolar adjunct (arrowheads) in Cetn1+/+ mice. (B) CETN1 was absent, centriole arrangement of the flagellar basal apparatus was disorganized and spermatogenesis was arrested in the last phase in Cetn1−/− mice. No CETN3 staining was identified in later steps of spermiogenesis (indicated by the X). (C) Scheme representing the polarization of CETN3/CETN1 during the acrosome phase; A, adjunct; dC, distal centriole; pC, proximal centriole; arrows and arrowheads as in A. Scale bars: 0.5 µm.