FIG. 5.

Overexpression of REF2 rescues the defects of swd2-3 and swd2-5. (A) Suppression of a thermosensitive growth defect. Cells were transformed with a 2μm high-copy-number plasmid carrying the REF2 gene under the control of a galactose-inducible promoter (pYES-REF2) or vector (pYES) alone. Serial dilutions of cells were spotted onto media containing galactose but lacking uracil and leucine and incubated at 30 or 37°C. (B) Effects of REF2 overexpression on the SNR13 termination defect. Northern blot analysis was performed as for Fig. 3E.