Fig. 2.
Palmitate (16:0) metabolism through the n-7 and n-9 pathways in 16HBE cells. Sense (wildtype, WT) and antisense (cystic fibrosis, CF) cells were cultured in complete medium for seven days and then incubated with 4.1 μM [1-14C]16:0 in reduced-lipid cell culture medium for 4 h and harvested (a) or washed twice in PBS and incubated for an additional 20 h in serum-containing medium (b). Levels of labeled 16:0, 16:1n-7, 18:0, and 18:1n-9 were determined by HPLC as described in “Materials and Methods”. Data are expressed as percentages of total counts and bars represent mean ± SEM (n = 3). The findings are representative of at least two independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 for sense versus antisense cells