Fig. 5.
Relative mRNA expression of metabolic enzymes in the n-7 and n-9 pathways. Sense (WT) and antisense (CF) cells (a) or C38 (WT) and IB3 (CF) cells (b) were incubated in complete medium for seven days, after which RNA was extracted and cDNA synthesized as described in “Materials and Methods”. qRT-PCR was performed using primers for the mRNA sequences of elongase 6 (ELOVL6) and Δ9-desaturase (SCD). Relative expression was determined by the ΔΔCT method using ribosomal protein RPLP0 as a control. Bars represent mean ± SEM (n = 3). The findings are representative of at least two independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 for sense versus antisense or IB3 versus C38 cells