Table 1.
The species/strains of bacteria that were evaluated for their susceptibility to PlyCM
| Species/Strain | Sensitivity |
|---|---|
| C. perfringens ATCC 13124 (type A, CPE−) | I (reference) |
| C. perfringens ATCC 3624 (type A, CPE−) | II |
| C. perfringens ATCC 12915 (type A, CPE+) | II |
| C. perfringens ATCC 12916 (type A, CPE+) | III |
| C. perfringens ATCC 12917 (type A, CPE+) | III |
| C. perfringens ATCC 12919 (type A, CPE+) | II |
| C. perfringens ATCC 3626 (type B) | I |
| C. perfringens ATCC 3628 (type C) | I |
| C. perfringens NCTC 8346 (type D) | II |
| C. perfringens ATCC 27324 (type E) | III |
| C. perfringens: 3 untyped human fecal isolates | Two—I |
| One—II | |
| C. perfringens: 12 untyped canine fecal isolates | Three—I |
| Seven—II | |
| One—III | |
| One—IV | |
| C. tetani ATCC 19406, C. septicum ATCC 12464, C. beijerinckii ATCC 8260 | III |
|
C. difficile ATCC 43593, C. difficile ATCC 700057, C. difficile ATCC 9689, C. histolyticum ATCC 19401, C. sordelli ATCC 9714, C. sporogenes ATCC 3584, C. bifermentans ATCC 638 |
IV |
|
S. pyogenes D471, S. agalactiae 090R, S. pneumoniae R36, S. mutans in-house strain, E. faecalis V583, E. faecium EFSK-2, S. aureus RN4220, S. epidermidis ATCC 12228, M. lysodeikticus ATCC 4698, L. monocytogenes HER1083, B. anthracis 222, B. cereus ATCC 14579, B. subtilis SL4, B. thuringiensis HD-73, B. megaterium in-house strain, G. vaginalis ATCC 14018, P. aeruginosa PAO1, E. coli TOP10 |
IV |
If known, the toxinotype and CPE-status (for toxinotype A) are listed for C. perfringens strains. Each bacteria was suspended in phosphate/citrate buffer (pH=6.4)+150 mM NaCl and exposed to three PlyCM-concentrations (11.5 nM, 115 nM, and 1.15 µM); 11.5 nM represents the lowest concentration that brought the host strain ATCC 13124 to baseline OD (see Figs. 4 and 7). After 1 h, the samples were visualized at ×1,000 magnification and the OD was measured
Each bacteria was assigned a rank based on the following semi-quantitative criteria: I 11.5 nM PlyCM induced complete lysis, or >9/10 cells had converted from rod-forms to spheroplasts, II 115 nM PlyCM lead to either preceding observation, III 1.15 µM PlyCM induced lysis/spheroplast conversion (complete or partial); IV no lysis or spheroplast conversion was observed after 1 h, even at 1.15 µM