FIG. 1.

Cold exposure promotes p38 MAPK phosphorylation, PGC-1α expression, and UCP1 transcription in IBAT. As described in Materials and Methods, IBAT was harvested from individual mice exposed to the cold to measure the following factors. (A) Phosphorylated p38 MAPK (p38-P) and total p38 MAPK (p38-T) in IBAT, measured by immunoblotting with specific antisera. (B) PGC-1α and UCP1 mRNA detected by Northern blotting. (C) PGC-1α protein assessed by immunoblotting with specific antisera; each lane represents the average of three mice per group. The results shown are from one of four independent experiments.