(A) Time course of FAS expression was performed during the expansion period. FAS expression was analyzed in triplicate via flow cytometry on cells in the early expansion and differentiation phase - days 0-8 post-expansion. (B) At 10 days post-expansion, control MP cells (C) or MP cells were exposed to 6BG, TMZ, or combination of 6BG/TMZ for 3 days. The percent of FAS positive and the relative density of FAS expression was determined via flow cytometry. (C) MP cells were exposed to 6BG, TMZ, or 6BG/TMZ for 3 days and cellular lysates analyzed via Western blot for c-FLIPshort and c-FLIP long. Flow cytometric data are expressed as the mean and standard deviation of triplicate data points for each group. Flow cyotmetric data and Western analysis were repeated twice with similar results.