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. Author manuscript; available in PMC: 2014 May 15.
Published in final edited form as: Clin Cancer Res. 2013 Mar 27;19(10):2699–2709. doi: 10.1158/1078-0432.CCR-12-2671

Figure 5. Relevance of FAS-mediated signaling in MP cells exposed to 6BG/TMZ.

Figure 5

(A) As a control for antibody-mediated signaling through FAS, Jurkat T cells were incubated in triplicate with agonistic (Ag), neutralizing (Neut), isotype (Iso) or combinations of antibodies for 24 hours and the percent of nonviable cells determined via Annexin V staining and flow cytometry. MP cells that had been expanded in culture for 10 days were treated with 6BG/TMZ for 3 days in the presence of combinations of agonistic (Ag), neutralizing (Neut), and isotype (Iso) antibodies. Antibody was added each day of the 3-day culture and nonviable cells determined via Annexin V staining and flow cytometry on day 4 post-treatment. (B) Caspase-8 activity was determined in lysates from control and 6BG/TMZ-treated cells in the +/- of a caspase-8 specific inhibitor. Data are representative of two independent experiments (A) or 3 independent experiments (B).