Fig. 5.

Immunostaining for hypoxia and VEGF release in ACM surrounded by acellular gel. (i) Hypoxia was confirmed by positive binding of antibody (brown) against pimonidazole, an exogenous hypoxia marker, and can be observed on day 14. Scale bar: black 1 mm; red, 200 μm. (ii, iii) VEGF release was confirmed by anti-VEGF staining (red), with DAPI counterstaining (blue) on (ii) day 14 and (iii) day 21. Scale bars correspond to 1 mm and 200 μm for the main display and higher magnification, respectively.