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. Author manuscript; available in PMC: 2013 Jul 15.
Published in final edited form as: Free Radic Biol Med. 2012 Feb 4;52(9):2047–2056. doi: 10.1016/j.freeradbiomed.2012.01.027

Figure 7. Impaired NF-κB activation in NOD.Ncf1m1J BM-Mϕ after poly(I:C)-stimulation.

Figure 7

(A) Representative Western blot of phospho-IκB-α (S32/S36) and total IκB-α from NOD and NOD.Ncf1m1J BM-Mϕ untreated or treated with 25 μg/ml of poly(I:C) for 30′. (B) Densitometry bar graph of the average of 3 independent experiments of phospho-IκB-α (S32/S36) expression in poly(I:C)-stimulated NOD and NOD.Ncf1m1J BM-Mϕ. The phospho-IκB-α (S32/S36) expression level in stimulated NOD BM-Mϕ was arbitrarily set as 1. (C) Representative Western blot of NF-κB p50 and actin from NOD and NOD.Ncf1m1J BM-Mϕ nuclear extracts after 25 μg/ml poly(I:C) treatment for 60′. (D) Densitometry bar graph of the average of 3 independent experiments of NF-κB p50 expression in poly(I:C)-stimulated NOD and NOD.Ncf1m1J BM-Mϕ. NF-κB p50 expression level in stimulated NOD BM-Mϕ was arbitrarily set as 1. The results are representative of three independent experiments. * p < 0.05 versus poly(I:C)-stimulated NOD BM-Mϕ.