Table 1.
Negative-ion ESI-MS of oligosaccharide obtained from enzymatic digestion and radical depolymerization of hyaluronan.
| Found ions (charge) |
Calculated Mol Weight (Da) |
Sequences | Theoretical Weight (Da) |
Ion strength (M) |
Retention time (min) |
|
|---|---|---|---|---|---|---|
| dp | Assignment | |||||
| Even number | ||||||
| 377.9 | 378.9 | 2 | ΔGlcA-GlcNAc | 379.1 | 7.2 | |
| 757.0 | 758.0 | 4 | ΔGlcA-GlcNAc-GlcA-GlcNAc | 758.2 | 11.2 | |
| 567.6 | 1137.2 | 6 | ΔGlcA-GlcNAc-(GlcA-GlcNAc)2 | 1137.3 | 18.1 | |
| 756.9 | 1515.8 | 8 | ΔGlcA-GlcNAc-(GlcA-GlcNAc)3 | 1516.4 | 23.0 | |
| 946.7 | 1895.4 | 10 | ΔGlcA-GlcNAc-(GlcA-GlcNAc)4 | 1895.6 | 25.8 | |
| 395.9 (−1) | 396.9 | 2 | GlcA-GlcNAc | 397.1 | 1.8 × 104 | 6.1 |
| 775.1 (−1) | 776.1 | 4 | GlcA-GlcNAc-GlcA-GlcNAc | 776.2 | 7.2 × 104 | 8.4 |
| 1154.1(−1) | 1155.1 | 6 | GlcA-(GlcNAc-GlcA)2-GlcNAc | 1155.3 | 3.0 × 104 | 13.8 |
| 576.4 (−2) | 1154.8 | 5.6 × 104 | ||||
| 766.0(−2) | 1534.0 | 8 | GlcA-(GlcNAc-GlcA)3-GlcNAc | 1534.5 | 6.1 × 104 | 19.8 |
| 955.9(−2) | 1913.8 | 10 | GlcA-(GlcNAc-GlcA)4-GlcNAc | 1913.6 | 7.2 × 104 | 23.9 |
| Odd number | ||||||
| 598.9 (−1) | 599.9 | 3 | GlcNAc-GlcA-GlcNAc | 600.2 | 7.0 × 104 | 6.1 |
| 571.9 (−1) | 572.9 | 3 | GlcA-GlcNAc-GlcA | 573.1 | 1.3 × 105 | 8.6 |
| 978.0 (−1) | 979.0 | 5 | GlcNAc-GlcA-GlcNAc-GlcA-GlcNAc | 979.3 | 1.2 × 104 | 8.6 |
| 951.0(−1) | 952.4 | 5 | GlcA-GlcNAc-GlcA-GlcNAc-GlcA | 952.4 | 5.2 × 104 | 15.3 |
| 474.2(−2) | 952.0 | 2.2 × 104 | ||||
| 677.9 (−2) | 1357.8 | 7 | GlcNAc-(GlcA-GlcNAc)2-GlcA-GlcNAc | 1358.4 | 5.2 × 104 | 13.8 |
| 664.4 (−2) | 1330.8 | 7 | GlcA-(GlcNAc-GlcA)2-GlcNAc-GlcA | 1331.4 | 1.1 × 105 | 20.9 |
| 867.8 (−2) | 1737.6 | 9 | GlcNAc-(GlcA-GlcNAc)3-GlcA-GlcNAc | 1737.5 | 4.0 × 104 | 19.6 |