FIG. 2.

In vitro cleavage efficiency of the mLPA is equivalent to that of WT SVLPA. (A) Linear sequence diagram of the construction of mLPA from SVLPA. (B) In vitro cleavage efficiency of mLPA in comparison to that of SVLPA. (C) RNA structure analysis of mLPA: C, control lane with substrate only; L, alkaline hydrolysis nucleotide ladder; S1, structure analysis with nuclease S1; V1, structure analysis with RNase V1; Pb, structure analysis with Pb²⁺; G, G sequence analysis with RNase T1. (D) Predicted alternative structures in the downstream region of mLPA. USEs, upstream efficiency elements; Subs., substrate; Prods., products.