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In vivo polyadenylation efficiency of mLPA and its mutant forms corresponds well to in vitro cleavage efficiencies. WT mLPA, various mutant mLPAs, and the SVLPA were used as the polyadenylation signals in luciferase reporter plasmids. The levels of luciferase activity were compared to the activity of mLPA, which was set at 100%. The in vitro cleavage activities from Fig. 3 are shown for comparison.
