| Mass Spectrometry Parameter | Setting | Comment |
| Source | ESI | Michrom CaptiveSpray |
| Ion Model | Positive or Negative | |
| Method Length | Variable (~40-60 min) | |
| Resolution | 60,000-100,000 | |
| Spray (kV) | 1.75 | |
| Tube Lens (V) | 130 | Target Compound Dependent |
| Capillary Temperature (°C) | 250 | May shorten life of spray tip |
| Capillary Voltage (V) | 50 | Target Compound Dependent |
| Data Type | Centroid | (for Profile data, see Discussion) |