FIGURE 5.

Effects of amino acid exchanges on the silica-targeting efficacies of Sil3-derived polypeptide segments T6 and T7. A, list of the wild type and mutated polypeptide sequences used for expression as GFP fusion proteins in T. pseudonana. The Silica targeting column indicates the approximate relative silica-targeting efficacies of the Sil3-derived segments. The higher the number of + signs, the higher the targeting efficacy. − indicates lack of silica targeting. B, confocal fluorescence microscopy images of individual live cells in girdle view expressing the indicated GFP fusion proteins. Each cell is a representative example of the cells in the corresponding transformant strain. Green color is indicative of GFP fusion proteins, and red color is caused by chloroplast autofluorescence. White bars: 2 μm.