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. 2013 May 21;288(28):20111–20120. doi: 10.1074/jbc.M113.465427

FIGURE 6.

FIGURE 6.

Sestd1 cooperates with Dact1 in the PCP pathway. A, Dact1 and Sestd1 synergistically activate the Rho GTPase. The SRE.L reporter and Renilla plasmids were co-transfected with Dact1, Sestd1, or Dact1–2A-Sestd1 plasmids in 3T3 cells, and luciferase activity was measured 24 h later. Specificity of reporter activation for Rho GTPase activity is confirmed with the Rho-specific inhibitor, C3 exoenzyme (last lane). B, activation of Rho requires Dact1-Sestd1 complex formation and the Sestd1 SEC14 domain. The SRE.L reporter and Renilla plasmids were co-transfected with Dact1, Sestd1, Dact1–2A-Sestd1, Dact1–2A-Sestd1(275–696), or Dact1(85–778)-2A-Sestd1 and luciferase assayed as in A. n.s., not significant (p > 0.05); *, p < 0.05; **, p < 0.01.