FIGURE 7.

Germband-restricted Pvf2 expression restores hemocyte invasive migration in pvf2–3 mutants. A brachyenteron (byn) pattern of Pvf2 expression is visualized by GFP (green in Merge). Hemocytes are visualized by antibody labeling of Pvr (red in Merge). The boxed regions were visualized at higher magnifications in the lower three panels. Scale bars represent 50 μm in whole embryo panels, 25 μm in A and B) and 15 μm in A′ and B′ panels. UAS-mCD8-GFP/+;pvf2–3,UAS-pvf2/pvf2–3;bynGAL4/+ (pvf2–3,byn>pvf2) embryos are shown. The dashed yellow line indicates the edge of the retracting germband. Arrowheads in A indicate hemocytes in the germband and at the site of invasion into the germband (A′). Arrowheads in B indicate hemocytes in the ventral posterior and in B′ hemocytes surrounding the developing hindgut.