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Reduction of benzamidoxime using cytochrome b5 type B wild type and variants. A, assay carried out with mARC1. B, assay carried out with mARC2. 120 pmol of molybdenum (mARC), 30–210 pmol of heme (CYB5B), and 6 pmol of FAD (CYB5R) were incubated with 3 mm benzamidoxime in 20 mm MES, pH 6.0, for 15 min under aerobic conditions. Data are means ± S.D. of one batch (CYB5B variants) or of four batches (CYB5B WT) each incubated in duplicates and measured twice by HPLC.
