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. 2013 May 28;288(28):20248–20260. doi: 10.1074/jbc.M113.455956

FIGURE 2.

FIGURE 2.

CaMKIIα regulates FA dynamics upstream of ICAP-1α. a, time lapse sequences of eGFP-VASP-expressing osteoblasts treated or not with KN-93 (10 μm). The arrows indicate the turnover of a single adhesion complex. Scale bar, 5 μm. b, Icap-1+/+ and Icap-1−/− osteoblasts expressing eGFP-VASP were treated or not with KN-93 (10 μm) for 2 h, and focal adhesion dynamics were recorded. Four parameters were measured: assembly and disassembly rates, adhesion steady state duration, and total lifetime. At least 20 cells and 10 FAs in each cell type were recorded. ***, p < 0.0001. c, Icap-1+/+ and Icap-1−/− osteoblasts spread on FN (10 μg/ml) and treated or not with KN-93 (10 μm) for 16 h were immunostained to visualize vinculin and activated β1 integrin (using 9EG7 antibody). Scale bar, 10 μm. d, quantification of FA numbers in Icap-1+/+ and Icap-1−/− osteoblasts spread on FN (10 μg/ml) and treated or not with KN-93 (10 μm) for 16 h. ***, p value < 0.0001. e, quantification of FA dynamics in Icap-1+/+, Icap-1−/−, and Icap-1WT osteoblasts expressing either eGFP- or mRFP-VASP and treated or not with KN-93 (10 μm) for 16 h. Three parameters were measured: assembly and disassembly rates and the adhesion lifetime. At least 20 cells and 10 FAs in each cell type were recorded. ***, p < 0.0001. Error bars, S.D.