FIGURE 3.

CaMKIIα regulates FA formation and dynamics through ICAP-1α binding to β1 cytodomain. a, β1^wild-type, β1^D759A, and β1^V787T osteoblast cells expressing CaMKIIα^T286D mutant were labeled with anti-activated β1 integrin and anti-CaMKIIα antibodies. Scale bar, 10 μm. b, quantification of β1 integrin-containing FA size in β1^wild-type, β1^D759A, and β1^V787T osteoblast cells expressing or not the constitutively activated CaMKIIα^T286D. ***, p < 0.0001. c, β1^wild-type, β1^D759A, and β1^V787T osteoblast cells were treated or not with 10 μm KN-93 for 2 h and immunostained to visualize vinculin and activated β1 integrin. Scale bar, 10 μm. d, quantification of β1 integrin-containing FA size in β1^wild-type, β1^D759A, and β1^V787T osteoblast cells treated or not with KN-93 (10 μm) for 2 h. ***, p < 0.0001. e, quantification of FA assembly and disassembly rates and total lifetime in β1^wild-type, β1^D759A, and β1^V787T osteoblast cells expressing eGFP-VASP and treated or not with 10 μm KN-93 for 16 h. ***, p value < 0.0001. Error bars, S.D.