Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 May 29;288(28):20267–20279. doi: 10.1074/jbc.M113.465369

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 1. — KEG interacts with ABI5 in the cytoplasm. A–D, localization of KEG-YFP (A), KEG^AA-YFP (B), and ABI5-CFP (C and D) in transiently transformed tobacco leaf epidermal cells. Right panels show fluorescence images from a combined series of Z-stack. Left panels show transmitted light images merged with fluorescence images from a single optical section. Bars = 100 μm. D displays an enlargement of the section delimited by a white square in C. Bar = 10 μm. E–G, localization of KEG-YFP (E), KEG^AA-YFP (F), and ABI5-CFP (G) in root cells of transgenic Arabidopsis seedlings. Right panels show fluorescence images from a single optical section. Left panels show transmitted light images merged with fluorescence images from a single optical section. Bars = 10 μm. H and I, BiFC analysis in tobacco epidermal cells. KEG-YN (H) or KEG^AA-YN (I) was coexpressed with ABI5-YC. Bars = 100 μm. J, levels of ABI5-YC following coexpression with KEG-YN or KEG^AA-YN. Analysis was done using protein extracts derived from the same samples assayed for BiFC shown in H and I. K, levels of ABI5-YC in tobacco epidermal cells coexpressing KEG-YN in the absence (−) and presence (+) of MG132. Note: ABI5-YC also contains a FLAG tag. Coomassie staining was used to confirm equal loading.