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. 2013 May 29;288(28):20267–20279. doi: 10.1074/jbc.M113.465369

FIGURE 2.

FIGURE 2.

ABI5 conserved C3 domain facilitates interactions with KEG ankyrin repeats. A and C, schematic representations of the KEG (A) and ABI5 (C) used in the yeast two-hybrid assays. Numbers indicate amino acids. B and D, yeast two-hybrid experiments showing interactions between ABI5 and KEG. Full-length ABI5 cDNA was fused with the GAL4-binding domain (BD) and KEG constructs (as illustrated in A) were fused with the Gal4 activation domain (AD) (B). Alternatively, ABI5 cDNA constructs (as illustrated in C) were fused to the GAL4-BD and KEG cDNA (ankyrin repeats, KEG497–829) was fused to the GAL4-AD (D). Interaction between ABI5 and KEG was analyzed by growth on selection medium without Trp, Leu, His, and Ade (SD/-T/-L/-H/-A). The pGADT7 and pGBKT7-Rec empty vectors were used as negative controls.