Cells transfected with CX50fs showed decreased intercellular transfer of Neurobiotin.
A, photomicrographs show intercellular transfer of Neurobiotin from the microinjected cell (*) to the neighboring cells in untransfected HeLa cells or in HeLa cells stably transfected with CX50, CX50fs, or CX50Tr. Scale bar, 161 μm. B, graph shows the quantification of these experiments. The results are presented as mean + S.E. (error bars; p < 10−8 for CX50 versus CX50fs or CX50Tr; p < 10−8 for untransfected versus any of the transfected cells).