FIGURE 4.

Epoxomicin dramatically increased levels of CX50fs. A, immunoblot shows the levels of immunoreactive CX50 in homogenates from HeLa-CX50 and HeLa-CX50fs cells left untreated or treated with DMSO (solvent for epoxomicin), 100 μm chloroquine, or 2 μm epoxomicin for 4 h. B, graph shows the quantification of the results obtained in three independent experiments performed as described in A. For each condition, the levels of CX50 and CX50fs were quantified by densitometry. For chloroquine, the -fold change in levels was calculated as the ratio between the densitometric values obtained after treatment with chloroquine and those obtained in Control. For epoxomicin, the -fold change in levels was calculated as the ratio between the densitometric values obtained after treatment with epoxomicin and those obtained in DMSO.