FIGURE 4.

Silencing of Csn8 caused an increased growth rate, whereas silencing of Csn5 impaired proliferation in HeLa cells. Dox-dependent vectors expressing small hairpin RNA targeting Csn8 (shCsn8) or Csn5 (shCsn5) were transfected to HeLa cells. A–D, growth curves of the cells containing pshCsn8 (A and B) or pshCsn5 (C and D) with or without induction by Dox were determined (A and C). Equal numbers of the cells taken from the growth curve analyses were lysed for Western blots for confirmation of Dox-dependent silencing of Csn8 (B) or Csn5 (D). Tubulin (anti-Tub) was used as loading control. The experiment was repeated three times.