FIGURE 5.

BAG6 nuclear localization and phosphorylation potentiate DNA damage induced cell death. A, U2OS cells and U2OS cells stably expressing the indicated proteins were treated with doxorubicin (2 μg/ml). Cell survival was determined using WST-1 assay at indicated times. B, U2OS cells stably expressing wild-type BAG6 (WT), BAG6 nuclear localization mutant (NLSmt), BAG6-S212A phospho-mutant (S212A) were transfected with an siRNA targeting the 3′-UTR region of BAG6 to knock down endogenous BAG6. Cells were treated with doxorubicin and assayed for survival as described in A. C, HeLa cells transfected with indicated siRNAs were treated with doxorubicin and assayed for cell survival at indicated times. All error bars represent mean ± S.D. (n = 3). *, p < 0.05; **, p < 0.01.